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KMID : 0604520090350030243
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Journal of the Society of Cosmetic Scientists of Korea
2009 Volume.35 No. 3 p.243 ~ p.250
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The Inhibitory Effects of Poria cocos Bark Extract on Melanogenesis
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Lee Eung-Ji
Bae Seong-Yun
Son Rak-Ho
Lee Yong-Hwa
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Abstract
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To develop a new natural whitening agent for cosmetics, we investigated the inhibitory effects of Poria cocos Bark extracts (PCBE) and its active compound on melanogenesis. PCBE showed ROS scavenging activities in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and xanthine/xanthine oxidase system with the IC50 values of 19.4 ± 2.21 μg/mL and IC50 = 103 ± 3.33 μg/mL, respectively. PCBE reduced intracellular tyrosinase activity about 34 % at concentration of 50 μg/mL. And PCBE reduced melanin contents of B16 melanoma cells about 51 % at concentration of 50 μg/mL without cell cytotoxicity (below 100 μg/mL). We purified one active compound from PCBE and identified its structure. It was identified as 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid, triterpene family, by 1H-NMR, 13C-NMR and Mass analysis. 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid showed ROS scavenging activities in DPPH radical and xanthine/xanthine oxidase system with the IC50 values of 4.3 ± 0.15 μg/mL and 54 ± 1.67 μg/mL, respectively. Also, it was shown that 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid reduced intracellular tyrosinase activity about 43 % at concentration of 10 μg/mL. And it inhibited melanin synthesis in a dose dependent manner (IC50 = 3.6 μg/mL) without cell cytotoxicity (below 100 μg/mL). 3-β-hydroxylanosta- 7,9(11),24-trien-4-oic acid inhibited tyrosinase, TRP-1 and TRP-2 expression at protein level. These results suggest that PCBE and 3-β-hydroxylanosta-7,9(11),24-trien-4-oic acid reduced melanin formation by the inhibition of tyrosinase activity and expression in B16 melanoma cells. Therefore, we suggest that PCBE could be used as a useful whitening agent.
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KEYWORD
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melanogenesis, Poria cocos Bark, whitening, 3-β-hydroxylanosta-7, 9(11), 24-trien-4-oic acid, tyrosinase
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